Choose program to use and database to search:
Program blastn blastp blastx tblastn tblastx Database Sp genome 4.2 Sp contigs 4.2 Sp genome 3.1 Sp Haplotype Specific contigs 1.0 Sp contigs 3.1 Sp genome 0.5 RNA-seq RepBase Sp genes Sp peptides RNA-seq peptide Clones Sp mRNAs common Trinity prediction Sp peptides common Trinity prediction Sp mRNAs predicted by evigene Sp peptides predicted by evigene
Enter here your input data as Sequence in FASTA format Accession or GI Or load it from disk
Set subsequence: From To
The query sequence is filtered for low complexity regions by default. Filter Low complexity Mask for lookup table only
Expect 0.0001 0.01 1 10 100 1000 Matrix PAM30 PAM70 BLOSUM80 BLOSUM62 BLOSUM45 Perform ungapped alignment
Query Genetic Codes (blastx only) Standard (1) Vertebrate Mitochondrial (2) Yeast Mitochondrial (3) Mold Mitochondrial; ... (4) Invertebrate Mitochondrial (5) Ciliate Nuclear; ... (6) Echinoderm Mitochondrial (9) Euplotid Nuclear (10) Bacterial (11) Alternative Yeast Nuclear (12) Ascidian Mitochondrial (13) Flatworm Mitochondrial (14) Blepharisma Macronuclear (15)
Database Genetic Codes (tblast[nx] only) Standard (1) Vertebrate Mitochondrial (2) Yeast Mitochondrial (3) Mold Mitochondrial; ... (4) Invertebrate Mitochondrial (5) Ciliate Nuclear; ... (6) Echinoderm Mitochondrial (9) Euplotid Nuclear (10) Bacterial (11) Alternative Yeast Nuclear (12) Ascidian Mitochondrial (13) Flatworm Mitochondrial (14) Blepharisma Macronuclear (15)
Frame shift penalty for blastx 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 25 30 50 1000 No OOF
Other advanced options:
Graphical Overview Alignment view Pairwise master-slave with identities master-slave without identities flat master-slave with identities flat master-slave without identities BLAST XML Hit Table Descriptions 0 10 50 100 250 500 Alignments 0 10 50 100 250 500 Color schema No color schema Color schema 1 Color schema 2 Color schema 3 Color schema 4 Color schema 5 Color schema 6